Survival of Candida albicans and Pseudomonas aeruginosa in oil polluted tropical coastal waters

TitleSurvival of Candida albicans and Pseudomonas aeruginosa in oil polluted tropical coastal waters
Publication TypeJournal Article
Year of Publication1998
AuthorsFuentes FA, Santo Domingo JW, Hazen TC
JournalWater Research
Volume32
Pagination2154-2170
Date PublishedJul
ISBN Number0043-1354
Accession NumberISI:000074735800021
KeywordsOil and Gas Degradation
AbstractThe effect of environmental abiotic factors on survival and activity of oil degrading isolates, Pseudomonas aeruginosa OD-1 and Candida albicans OD-2, was determined in situ using membrane diffusion chambers. The study sites were located in a tropical bay on the Atlantic Ocean with history of petroleum contamination by oil tankers. Microbial densities were measured by direct counts. H-3-thymidine uptake, microautoradiography, INT-reduction, adenosine triphosphate (ATP), and frequency of dividing cells were used to assess microbial activity. Both organisms showed a significant decrease in cell density over a three day period, although, temporal increases in densities were observed. Significant decreases in total activity vr;ere observed ibr both populations during the study; however, the respiration potential and ATP content per organism remained constant or even increased by the end of the study suggesting that a significant fraction of these populations were capable of withstanding in situ environmental conditions. Results suggest that increases in ambient phosphorus concentrations played a role in the prolonged ill situ survival of these petroleum degrading isolates at some sites. The traffic of oil tankers might have played a significant impact on microbial survival in this bay, as violent agitation of sediments increased phosphorus concentrations available to microorganisms. Published by Elsevier Science Ltd.