MEASURING THE POTENTIAL ACTIVITY OF HYDRO CARBON DEGRADING BACTERIA

TitleMEASURING THE POTENTIAL ACTIVITY OF HYDRO CARBON DEGRADING BACTERIA
Publication TypeJournal Article
Year of Publication1976
AuthorsWalker JD, Colwell RR
JournalApplied and Environmental Microbiology
Volume31
Pagination189-197
ISBN Number0099-2240
Accession NumberBIOSIS:PREV197662007827
KeywordsOil and Gas Degradation
Abstract[14C]hydrocarbons were utilized to estimate the hydrocarbon-degrading potential of bacteria in estuarine and marine environments. Evaporation of the hydrocarbons must be considered in estimates of oxidation. Amount of mineralization of [14C]hexadecane can be equated with the total number of petroleum-degrading bacteria and the percentage of the total heterotrophic population which they represent. Mineralization activity was related to the activity of the bacterial populations during in situ incubation. Rates of mineralization were [14C]hexadecane > [14C]naphthalene > [14C]toluene > [14C]cyclohexane. Increased rates of uptake and mineralization were observed for bacteria in samples collected from an oil-polluted harbor compared with samples from a relatively unpolluted, shellfish-harvesting area, e.g., turnover times of 15 and 60 min for these areas, respectively, using [14C]hexadecane.