Characterization of native fungi responsible for degrading crude oil from the coastal area of Yanbu, Saudi Arabia

TitleCharacterization of native fungi responsible for degrading crude oil from the coastal area of Yanbu, Saudi Arabia
Publication TypeJournal Article
Year of Publication2017
AuthorsEl-Hanafy A.A, Anwar Y., Sabir J.SM, Mohamed S.A, Al-Garni S.MS, Zinadah O.AHAbu, Ahmed M.M
JournalBiotechnology & Biotechnological Equipment
Volume31
Pagination105-111
ISBN Number1310-2818
Accession NumberWOS:000390379500015
KeywordsOil and Gas Degradation
AbstractA total of 15 fungal isolates were obtained from oil-contaminated sites near the Red Sea in the Yanbu region. Based on the preliminary DCPIP (2,6-dichlorophenolindophenol) assay, three isolates showed promising oil degrading ability. The next-generation sequencing of the ITS-I and ITS-II internal transcribed spacer regions assigned the isolates to Aspergillus and Penicillium. Among these three strains, Y2 (Aspergillus oryzae) was the most efficient, degrading about 99% of the crude oil. The degradation rates were corroborated using spectrophotometric and gas chromatographymass spectrometry analyses after two weeks of cultivation in Bushnell-Haas medium. All the three strains proved to be potent oil-degrading strains and, hence, can be utilized to degrade oil contaminants.
DOI10.1080/13102818.2016.1249407